Problem Statement:
Showing multiple overlays in single view using WPF Prism region manager and regions
Solution:
Have region using ItemsControl or Listbox
Listbox XAML Sample
ItemsControl XAML Sample
Bind your regions with multiple views to show as overlay
regionManager.Regions[MultipleOverlayRegion].Add(overlayView1);
regionManager.Regions[MultipleOverlayRegion].Add(overlayView2);
Showing multiple overlays in single view using WPF Prism region manager and regions
Solution:
Have region using ItemsControl or Listbox
Listbox XAML Sample
     <ListBox x:Name=" MultipleOverlayRegion "
                         PrismRegions:RegionManager.RegionName="MultipleOverlayRegion">
            <ListBox.ItemsPanel>
                <ItemsPanelTemplate>
                    <Grid></Grid>
                </ItemsPanelTemplate>
            </ListBox.ItemsPanel>
            <ListBox.ItemTemplate>
                <DataTemplate>
                    <Grid>
                        <ContentControl Content="{Binding}" />
                    </Grid>
                </DataTemplate>
            </ListBox.ItemTemplate>
            <ListBox.ItemContainerStyle>
                <Style TargetType="ListBoxItem"
               BasedOn="{StaticResource {x:Type ListBoxItem}}">
                    <Setter Property="VerticalContentAlignment"
                    Value="Stretch" />
                    <Setter Property="Template">
                        <Setter.Value>
                            <ControlTemplate TargetType="ListBoxItem">
                                <ContentPresenter />
                            </ControlTemplate>
                        </Setter.Value>
                    </Setter>
                </Style>
            </ListBox.ItemContainerStyle>
        </ListBox>
  <ItemsControl x:Name="MultipleOverLayRegion">
            <ItemsControl.ItemsPanel>
                <ItemsPanelTemplate>
                    <Grid></Grid>
                </ItemsPanelTemplate>
            </ItemsControl.ItemsPanel>
  </ItemsControl>
regionManager.Regions[MultipleOverlayRegion].Add(overlayView1);
regionManager.Regions[MultipleOverlayRegion].Add(overlayView2);
 
